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KMID : 0613820160260040460
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Journal of Life Science
2016 Volume.26 No. 4 p.460 ~ p.467
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Characterization of a Korean Domestic Cyanobacterium Limnothrix sp. KNUA012 for Biofuel Feedstock
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Hong Ji-Won
Jo Seung-Woo
Kim Oh-Hong
Jeong Mi-Rang
Kim Hyun
Park Kyung-Mok
Lee Kyoung-In
Yoon Ho-Sung
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Abstract
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A filamentous cyanobacterium, Limnothrix sp. KNUA012, was axenically isolated from a freshwater bloom sample in Lake Hapcheon, Hapcheon-gun, Gyeongsangnam-do, Korea. Its morphological and molecular characteristics led to identification of the isolate as a member of the genus Limnothrix. Maximal growth was attained when the culture was incubated at 25°C. Analysis of its lipid composition revealed that strain KNUA012 could autotrophically synthesize alkanes, such as pentadecane (C15H32) and heptadecane (C17H36), which can be directly used as fuel without requiring a transesterification step. Two genes involved in alkane biosynthesis－an acyl-acyl carrier protein reductase and an aldehyde decarbonylase－were present in this cyanobacterium. Some common algal biodiesel constituents―myristoleic acid (C14:1), palmitic acid (C16:0), and palmitoleic acid (C16:1)－were produced by strain KNUA012 as its major fatty acids. A proximate analysis showed that the volatile matter content was 86.0% and an ultimate analysis indicated that the higher heating value was 19.8 MJ kg-1. The isolate also autotrophically produced 21.4 mg g-1 phycocyanin－a high-value antioxidant compound. Therefore, Limnothrix sp. KNUA012 appears to show promise for application in cost-effective production of microalga-based biofuels and biomass feedstock over crop plants.
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KEYWORD
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Alkane-producing genes, biofuel, cyanobacteria, phycocyanin, potential feedstock
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